WebbHeparinase (200 U/mL; Thermo Fisher Scientific) treatment was conducted for 3 h at 25°C by mixing 10 µL of obtained RNA samples with 10 µL of heparinase solution. Heparinase-treated total RNA samples were used for cDNA synthesis using the miRCURY RT kit (Qiagen). Reaction conditions were set according to recommendations by the … WebbThe Thermo Scientific TMT Comm communication software is designed to communicate with the Thermo Scientific series of density and level gauges via the RS485 serial port. …
IGFBP2 function as a novel biomarker for active lupus nephritis
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133077060444232040 - lecture notes - Studocu
Webb28 juni 2016 · For all three purification kits the manufactures instructions were followed after pre-treatment. DNA was eluted using 40 µl NFW and stored at −20 °C. The concentration of the RNA and DNA samples were assessed using NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). WebbThe flow-through, containing TMT-blocked N-terminal peptides, was desalted using C18 columns (Hypersep, Thermo, Waltham, MA, USA). The sample was pre-fractionated on C18 Hypersep columns using 15%, 25% and 60% acetonitrile in 1% ammonium hydroxide as elution buffers, respectively. Webb14 mars 2024 · Specific sandwich ELISA kits were used to detect the ... a p value <0.05 according to DAVID and containing at least 10 genes per pathway were reported. The Tandem mass tag (TMT) proteomic ... formic acid in water. Shotgun proteomics was performed using an EASY-nLCTM 1200 UHPLC system (Thermo Fisher) coupled with a Q … results is consistent with macroamylasemia