Addgene zeocin
WebZeocin-, G418-, nourseothricin- and blasticidin-resistance genes are the only dominant selectable markers currently available for selecting P. pastoris transformants. We describe here new P. pastoris expression vectors that confer a hygromycin resistance base on the Klebsiella pneumoniae hph gene. WebLearn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Zeocin® is an InvivoGen trademark. How to cite this plasmid ( Back to top) These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were ...
Addgene zeocin
Did you know?
WebLearn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Zeocin® is an InvivoGen trademark. How to cite this plasmid ( Back to top) These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were ... WebThe Zinc Finger Consortium was established to promote continued research and development of engineered zinc finger technology. Consortium members Keith Joung …
Web• Zeocin resistance gene for selection in both E. coli and Pichia • C-terminal peptide containing the c-myc epitope and a polyhistidine (6xHis) tag for detection and purification of a recombinant fusion protein For Research Use Only. Not for use in diagnostic procedures. Specifications Promoter AOX1 Product Type Pichia Expression Vector WebThe following protocol outlines the steps to generate kill curve for your cells. What you need: Cell line of your interest in healthy condition Complete culture medium (medium + serum) recommended for your cell line Selection antibiotic of your interest Steps: Harvest healthy adherent cells either by using trypsin or by gentle cell scraping.
WebZeocin Phleomycin D1 Resistance Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … WebZeocin® is an InvivoGen trademark. How to cite this plasmid ( Back to top ) These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the … Zeocin® is an InvivoGen trademark. Depositor Comments. Constructs for … Learn more about Addgene materials from user-contributed reports describing AAV …
Web• Zeocin™ or hygromycin resistance gene for effective selection of stable mammalian cell lines • Large multiple cloning site to simplify cloning
WebAug 30, 2024 · The first step is to performe a dose response to know which zeocin concentration is lethal for your cells. I work with a concentration of 300μg / mL and the untransformed cells die after 48 hours... tomah post officeWebObtaining Zeocin™ ™Zeocin may be obtained from Invitrogen. For your convenience, the drug is prepared in autoclaved, deionized water and available in 1.25 ml aliquots at a concentration of 100 mg/ml. The stability of Zeocin™ is guaranteed for six months if stored at –20°C. Amount Catalog no. 1 g R250-01 5 g R250-05 Accessory Products tomah police department tomah wiWebThe pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli. The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines. For Research Use Only. Not for use in diagnostic procedures. people鈥檚 medical publishing houseWebLearn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Zeocin® is an InvivoGen trademark. How … peopling of south asia bioessays 2007WebZeocin™ Selection Antibiotic is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures. It is used at a working concentration ranging between 50–400 … tomah pharmaciesWebAddgene is a non-profit plasmid repository. Addgene facilitates the exchange of genetic material between laboratories by offering plasmids and their associated cloning data to … tomah police department facebookWebMar 16, 2024 · The product of Zeocin R binds to Zeocin in a one-to-one ratio and prevents the DNA cleavage. Zeocin R was used as the selection marker owing to its relatively smaller size (375 bp versus 1 kb for the hygromycin resistance gene) and greater reliability in stable cell clone selection ( 19 ). people怎么读音发音